首页 |  站点地图 |  我的农科院 |  ENGLISH
 
  首页 -> 论文
 论文题目   Characterizing the expression of translation elongation factor gene EF1α, in pear (Pyrus ) fruit: evaluation of EF1α, as a housekeeping gene 
 第一作者   Wang Y
 发表年份   2018
 发表刊物与期数  《  Tree Genetics & Genomes 》4期14卷
 起止页码   62
 全部完成人   Wang Y, Dai M, Cai D, Miao L(苗立祥), Wei L, Shi Z
 论文摘要   Reference genes are a key factor for the sensitivity and reliability of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. The elongation factor gene EF1α encodes a highly conserved ubiquitous protein that functions in the binding of aminoacyl-tRNA to the ribosome during peptide synthesis in eukaryotes, which is proposed for qRT-PCR as an internal reference for its putative housekeeping gene character. However, information about the paralogous copies and expression diversification of EF1α has been neglected and ill defined. In this study, the members of pear (Pyrus) EF1α were explored by mining public data. The family- and/or species-specific members of EF1α in Rosaceae plants or other species revealed by phylogenetic analysis suggested that the specific gene expansion occurred after the family or species diversification. The expression analysis based on the high-throughput sequencing and sequence differences typing provided a high resolution to distinguish the expression among the members of pear EF1α. The EF1α members showed an obviously unstable expression in both pear and apple (Malus × domestica) fruits at different developmental stages, clustered by two conserved expression patterns in pear fruit. The complementary expression among certain pear EF1α members leads to the sum expression of these members having a higher level of expression stability among the fruit at different developmental stages, which can be used as an approach to optimize EF1α as the internal reference. The result can also provide insight into the expression characteristics of pear EF1α in other tissues of pear. In addition, it indicates that any two genes having complementary expression patterns can be used as a reference for qPCR analysis with the high stability of their mean expression value.
 关键词   Gene expression; mRNA sequencing ; qRT-PCR; Reference gene
 影响因子   1.829
 ISSN   1614-2942
  您是第 
  个访问本站的客人!  
浙ICP备09030064号  |  浙江省农业科学院.  All Rights Reserved. 杭州市德胜中路298号, 310021. (0571) 86404011.